Novel features of monocytes and macrophages in cardiovascular biology and disease. Biology and therapeutic targeting of tumour-associated macrophages. Recent advancements in biopolymer and metal nanoparticle-based materials in diabetic wound healing management. Vijayakumar V., Samal S.K., Mohanty S., Nayak S.K. Non-healing foot ulcers in diabetic patients: general and local interfering conditions and management options with advanced wound dressings. Uccioli L., Izzo V., Meloni M., Vainieri E., Ruotolo V., Giurato L. Understanding the multifaceted mechanisms of diabetic wound healing and therapeutic application of stem cells conditioned medium in the healing process. Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing. Patel S., Srivastava S., Singh M.R., Singh D. Chronic complications of Diabetes Mellitus: A mini review. Lotfy M., Adeghate J., Kalasz H., Singh J., Adeghate E. Therefore, these findings demonstrates that PAQR3 silencing accelerates diabetic wound healing by promoting M2 macrophage polarization and angiogenesis, which is mediated by the inhibition of STUB1-mediated PPARγ protein ubiquitination and degradation. The PPARγ inhibitor, GW9662, or STUB1 overexpression abrogated the enhanced M2 macrophage polarization induced by PAQR3 silencing. STUB1 (STIP1 homology and U-box-containing protein 1) binds with the PPARγ protein to mediate PPARγ ubiquitination and degradation in macrophages, which was impaired by PAQR3 silencing. The ubiquitination of PPARγ protein in macrophages was repressed by PAQR3 silencing. Moreover, knockdown of PAQR3 in macrophages enhanced the migration of HaCaT cells and tube formation of HUVECs. PAQR3 silencing also promoted M2 polarization and increased PPARγ protein level in PMA-treated THP-1 cells. We showed that PAQR3 silencing promoted skin wound healing and angiogenesis in diabetic mice, which was accompanied by enhanced M2 macrophage polarization and elevated expression of PPARγ (peroxisome proliferator-activated receptor γ). In this study, we aimed to investigate the potential effects of PAQR3 (progestin and adipoQ receptor 3) silencing in accelerating diabetic wound healing. However, the underlying mechanism remains poorly understood. The pathogenesis of diabetic wounds is closely associated with the dysregulation of macrophage polarization.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |